Peroxidase (POD, EC 1.11.1.7) widely exists in animals, plants and microorganisms. It can catalyzes
the oxidation of phenols and amines by hydrogen peroxide, and has the dual effect of eliminating toxicity
of hydrogen peroxide, phenols and amines. In the presence of hydrogen peroxide, POD can catalyzes
H2O2 oxidize specific substrates to produce one substance which has a absorption at 470 nm.
Reagents and Equipment Required but Not Provided:
Spectrophotometer/microplate reader, desk centrifuge, transferpettor, micro glass cuvette/96-well flat-
bottom plates, mortar//homogeniser, ice and distilled water.