6-phosphate dehydrogenase (G6PDH) is widely found in animals, plants, microorganisms and cultured
cells. It is a key enzyme in the pentose phosphate pathway, which catalyzes the oxidation of glucose 6-
phosphate to glucose 6-phosphate lactone. In the meantime, G6PDH reduces NADP+ to NADPH, which
provides biosynthesis and maintain the reduction state of cells. The activity of G6PDH can reflect the
biosynthesis level and antioxidant capacity of the organisms.
G6PDH catalyzes the conversion of NADP+ to NADPH. In this kit, the activity of G6PDH is determined
by the increase rate of NADPH at 340 nm.
Reagents and Equipment Required but Not Provided:
Spectrophotometer/microplate reader, micro quartz cuvette/96 well UV flat-bottom plate, water bath,
refrigerated centrifuge, adjustable transferpettor, mortar/ homogenizer, ice and distilled water.
