PDH is widely exist in animals, plants, microorganism and cultured cells, which is the rate-limiting
enzyme of acetylformic acid oxidative and decarboxylate catalyzed by Pyruvate dehydrogenase complex
(PDHC). The decarboxylation of acetylformic acid forms hydroxyethyl -TPP, links glycolysis to the three
carboxylic acid cycle.
PDH catalyzes the dehydrogenation of acetylformic acid and reduct 2, 6-dichlorophenol indophenol (2,6-
DCPIP), which makes the absorption of 605 nm decrease.
Reagents and Equipment Required but Not Provided:
Spectrophotometer, water bath, desk centrifuge, adjustable pipette,1 mL glass cuvette,
mortar/homogenizer, ice and distilled water.
