The activity of soil dehydrogenase can reflect the amount of active microorganisms in the soil systemthe
and its degradation activity to organic compounds, it can be used as the degradation index of soil
microorganisms.
The hydrogen receptor 2, 3, 5-triphenyl tetrazolium chloride (TTC) is reduced to Triphenyl Formazone
(TF) during cellular respiration, TF is red which has a maximum absorption peak at the wavelength of 485
nm. Detecting the absorbance at 485 nm can obtain the activity of soil dehydrogenase.
Required reagents and equipment:
Sieve, scale, constant temperature incubator/water bath, centrifuge, funnel, filter paper,
Spectrophotometer/Microplate reader, micro glass cuvette/96 well flat-bottom plate, ice, distilled water,
acetone(self-provided).
