ADPG Pyrophosphorylase(AGP) Activity Assay Kit

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ADPG焦磷酸化酶(AGP)活性检测试剂盒

Cat:

NA0591

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/96S

Storage instructions：

-20℃

Product Overview：

Components:

Extract solution: 100 mL×1. Store at 4℃.

Reagent I: 20 mL×1. Store at 4℃.

Reagent II: Powder×1. Store at -20℃. Dissolve with 6.4 mL of distilled water before use. Unused reagent

is still stored at -20℃.

Reagent III: Powder×2. Store at 4℃. Dissolve with 2 mL of distilled water before use. Unused reagent is

stored at -20℃.

Reagent IV: Powder×2. Store at -20℃. Dissolve with 500 μL of distilled water before use. Unused reagent

is still stored at -20℃.

Reagent V: 250 μL×2. Store at -20℃.

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Unit:

Price: $770

Product PDFs

Datasheet:

ADPG Pyrophosphorylase(AGP) exists mainly in plants, is the main rate-limiting step in plant starch

biosynthesis, which catalyzes the reaction of glucose-1-phosphate (G1P) with ATP to produce direct

precursor adenosine diphosphate glucose (ADPG) for starch synthesis.

AGP catalyzes the reverse reaction to produce G1P, the added phosphate hexose mutase and 6-phosphate

glucose dehydrogenase catalyze the formation of 6-phosphate gluconate and NADPH. In this kit, the

activity of AGP is determined by the increase rate of NADPH at 340 nm.

Reagents and Equipment Required but Not Provided:

Spectrophotometer/Microplate Reader, desk centrifuge, adjustable pipette, water bath, micro quartz

cuvette/96 well flat-bottom UV plate, mortar/homogenizer, ice, distilled water.

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