Phosphofructokinase(PFK) Activity Assay Kit

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磷酸果糖激酶(PFK)活性检测试剂盒

Cat:

NA0827

Assay Type:

Spectrophotometer

Brand:

sunlong medical

Specificity:

50T/48S

Storage instructions：

-20℃

Product Overview：

Components:

Extract Solution: Liquid 60 mL×1. Storage at 4℃.

Reagent I: Liquid 40 mL×1. Storage at 4℃.

Reagent Ⅱ: Powder ×1. Storage at -20℃. Add 2.8 mL of distilled water before use and dissolve it fully.

Reagent Ⅲ: Liquid45μL ×1. Storage at -20℃. The Liquid is placed in the EP tube in the reagent bottle.

According to the volume ratio of Reagent Ⅲ: distilled water is 2:13, mix well before use. prepared when

the solution will be used; it is recommended to pack and store the unused Reagent Ⅲ solution at -20℃ to

avoid repeated freezing and thawing;

Reagent Ⅳ: Liquid 20μL ×1. Storage at 4℃. The liquid is placed in the EP tube in the reagent bottle.

According to the volume ratio of Reagent Ⅲ: distilled water is 4:65, mix well before use. prepared when

the solution will be used; and the mixture is ready to use;

Preparation of PFK working fluid (25 samples can be measured): Take 19 mL of Reagent I and 1.26 mL of

Reagent Ⅱ and mix them thoroughly

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Unit:

Price: $154

Product PDFs

Datasheet:

PFK (EC 2.7.1.11) is one of the key regulatory enzymes in the process of glycolysis, which widely found

in animals, plants, microorganisms and cultured cells. It is responsible for converting fructose-6-phosphate

and ATP into fructose-diphosphate and ADP.

PFK catalyzes the formation of fructose-1,6-diphosphate and ADP to from fructose-6-phosphate and ATP.

Pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to generate NAD⁺. The

degradation rate of NADH which measured at 340 nm is used to reflect the activity of PFK.

Reagents and Equipment Required but Not Provided.

Table centrifuge, water-bath, UV spectrophotometer, 1 mL quartz cuvette, adjustable pipette,

mortar/homogenizer, ice and distilled water.

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