Triglyceride(TG) is a fat molecule formed by long-chain fatty acids and glycerol, which is not only the
main component of cell membrane, but also an important respiratory substrate. The TG is extracted with
isopropyl alcohol, then hydrolysis to glycerol and fatty acids after saponification of TG by KOH. Glycerol
is oxidized by periodic acid to form formaldehyde. Condensation of formaldehyde and acetylacetone to
form yellow components in presence of chloride ions. The yellow component has a characteristic
absorption at 420 nm and proportional to the TG content.
Reagents and Equipment Required but Not Provided:
Spectrophotometer, water bath, adjustable pipette, 1 mL glass cuvette, n-heptane, isopropyl alcohol,
distilled water, 100 mL empty glass bottle.