MDHAR catalyzes MDHA to form AsA, which plays an important role in ascorbic acid redox metabolism.
NADH reduces MDHA to generate AsA and NAD+ under the conditions of MDHAR catalysis. NADH has
a characteristic absorption peak at 340 nm, but NAD+ is not. The activity of MDHAR can be calculated by
measuring the decrease rate of absorption at 340 nm.
Reagents and Equipment Required but Not Provided:
Mortar/homogenizer, ice, desk centrifuge, spectrophotometer, 1 mL quartz cuvette, adjustable pipette,
distilled water.
