Monodehydroascorbate Reductase(MDHAR) Activity Assay Kit

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单脱氢抗坏血酸还原酶(MDHAR)活性检测试剂盒

Cat:

NA0575

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/96S

Storage instructions：

-20℃

Product Overview：

Components:

Extract solution: 110 mL×1. Storage at 4℃.

Reagent I: 12 mL×1. Storage at 4℃.

Reagent II: Powder×1. Storage at 4℃, protected from light. Dissolve thoroughly with 2 mL of distilled

water when the solution will be used.

Reagent III: Powder×1. Storage at -20℃, protected from light. Dissolve thoroughly with 3.33 mL of

distilled water when the solution will be used.

Reagent IV:liquit×1. Storage at -20℃, protected from light. Dissolve thoroughly with 2 mL of Reagent I

when the solution will be used.

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Unit:

Price: $350

Product PDFs

Datasheet:

MDHAR catalyzes MDHA to form AsA, which plays an important role in ascorbic acid redox metabolism.

NADH reduces MDHA to generate AsA and NAD⁺under the conditions of MDHAR catalysis. NADH has

a characteristic absorption peak at 340 nm, but NAD⁺is not. The activity of MDHAR can be calculated by

measuring the decrease rate of absorption at 340 nm.

Reagents and Equipment Required but Not Provided:

Mortar/homogenizer, ice, desk centrifuge, spectrophotometer/microplate reader, micro quartz cuvette/96

well flat-bottom UV plate, adjustable pipette and distilled water.

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