Lactate dehydrogenase (LDH or LD) is the terminal enzyme of the glycolysis pathway which is found in
nearly all living cells (animals, plants, and prokaryotes). LDH catalyzes the conversion of lactate to
pyruvic acid and back, as it converts NAD+ to NADH and back.
NAD+ and lactic acid are oxidized to pyruvic acid by the catalysis of LDH. Pyruvate further reacted with
2,4-dinitrophenylhydrazide to form pyruvate dinitrobenzone, which show brown red color in alkaline
solution and the color depth is proportional to the concentration of pyruvate.
Reagents and Equipment Required but Not Provided:
Spectrophotometer/Microplate reader, thermostat water bath, desk centrifuge, adjustable pipette, micro
glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice, distilled water.
