Pyruvate Carboxylase(PC) Activity Assay Kit

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丙酮酸羧化酶（PC）活性检测试剂盒

Cat:

NA0810

Assay Type:

Spectrophotometer

Brand:

sunlong medical

Specificity:

50T/48S

Storage instructions：

-20℃

Product Overview：

Components:

Extract solution: Liquid 110 mL×1. Storage at 4℃.

Reagent I: Liquid 30 mL×1. Storage at 4℃.

Reagent II: Liquid 10 mL×1. Storage at 4℃.

Reagent III: Powder×1. Storage at -20℃. Dissolve with 5 mL of distilled water, store at -20℃ after

prepared.

Reagent IV: Powder×1. Storage at -20℃. Dissolve with 5 mL of distilled water, store at -20℃ after

prepared.

Reagent V: Liquid 5 mL×1. Storage at 4℃.

Reagent VI: Liquid 15 μL×1. Storage at 4℃.

Reagent VI Diluent Solution: Liquid 10 mL×1. Storage at 4℃.

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Unit:

Price: $282

Product PDFs

Datasheet:

Pyruvate carboxylase (PC, EC 6.4.1.1) is widely present in mitochondria of animals, molds and yeast, but

is not found in plants and most bacteria. PC is the main postreaction for oxaloacetate, and is the first rate-

limiting enzyme in the gluconeogenesis process.

PC irreversibly catalyzes pyruvate, ATP, CO2 and water to oxaloacetate, ADP and Pi, malic

dehydrogenase further catalyzes the formation of malic acid and NAD+ from acetoacetic acid and NADH.

The enzyme activity of PC can be reflected by detecting the oxidation rate of NADH at 340 nm.

Reagents and Equipment Required but Not Provided:

Ultraviolet spectrophotometer, water bath, desk centrifuge, water bath, adjustable pipette, 1 mL quartz

cuvette, mortar/homogenizer, ice and distilled water.

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