Fructose 1,6-bisphosphatase(FBP) also known as fructose-1,6-diphosphatase, which plays a key role in the
gluconeogenesis and the synthesis of photosynthetic assimilate sucrose.
FBP catalyzes fructose 1,6-diphosphate and water to produce 6-phosphate fructose and inorganic
phosphorus. Glucose-phosphate isomerase and 6-glucose-phosphate dehydrogenase added to the reaction
system that catalyze the formation of 6-glucose-phosphate gluconic acid and NADPH in turn. In this kit,
the activity of FBP is determined by the increase rate of NADPH at 340 nm.
Reagents and Equipment Required but Not Provided:
Ultraviolet spectrophotometer, desk centrifuge, adjustable pipette, water bath, 1 mL quartz cuvette,
mortar/homogenizes, ice and distilled water.