Pyruvate Decarboxylase(PDC) Activity Assay Kit

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丙酮酸脱羧酶(PDC)活性检测试剂盒

Cat:

NA0791

Assay Type:

Spectrophotometer

Brand:

sunlong medical

Specificity:

50T/48S

Storage instructions：

-20℃

Product Overview：

Components:

Extract solution: 60 mL×1. Storage at 4℃.

Reagent IA: 28 mL×1. Storage at 4℃.

Reagent IB: Powder×1. Storage at -20℃.

Reagent IC: 2 mL×1. Storage at 4℃.

Reagent IIA: 7 mL×1. Storage at 4℃.

Reagent IIB: Powder×1. Storage at -20℃.

Reagent IIC: Powder×1. Storage at -20℃.

Reagent III: 20 mL×1. Storage at 4℃.

Preparation of solution：

Extract solution: Contains insoluble substance. Shake well before use.

Preparation of Reagent I: Add reagent 1 B and reagent 1 C to reagent 1 A and dissolve thoroughly before

use. Separately store at -20℃ for 1 month.

Reagent IIA: Add 0.6ml distilled water to dissolve the reagent before use, and store the inexhaustible

reagent separately at -20℃ for two weeks.

Reagent IIB: Add 1ml distilled water to dissolve the reagent before use, and store the inexhaustible reagent

separately at -20℃ for two weeks.

Preparation of Reagent II: 2.625ml of reagent A, 0.225ml of reagent B and 0.15ml of reagent C were

mixed (3mL in total, about 30T) before use.

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Unit:

Price: $165

Product PDFs

Datasheet:

Other Information
Citations
Protein Attributes
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Pyruvate Decarboxylase (PDC) exists in yeast mainly, which is one of the key enzymes in ethanol

fermentation. PDC catalyzes pyruvate decarboxylation to form acetaldehyde. Ethanol dehydrogenase

(ADH) is added to further catalyze the reduction of acetaldehyde by NADH to produce ethanol and NAD⁺.

NADH has a absorbance at 340 nm but NAD not, the activity of PDC can be calculated by measuring

decrease rate of absorption at 340 nm.

Reagents and Equipment Required but Not Provided:

Spectrophotometer, water-bath, desk centrifuge, adjustable pipette, 1 mL quartz cuvette, mortar/

homogenizer, ice and distilled water.

Protocol:

I. Sample extraction:

1. Bacteria:

Suggested 5 million bacteria/cell with 1 mL of Extract solution. Splitting bacteria and cells with ultrasonic

(ice bath, power 20%, work time 3s, interval 10s, repeat for 30 times). Centrifuge at 16000×g for 20

minutes at 4℃, take the supernatant and place it on ice for test.

2. Tissue:

Add 1 mL of Extract solution into 0.1 g of tissue, fully grinding on ice. Centrifuge at 16000 ×g for 20

minutes at 4℃, take the supernatant and place it on ice for test.

3. Serum:

Detect directly.

II.

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