Pyruvate Decarboxylase(PDC) Activity Assay Kit

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丙酮酸脱羧酶(PDC)活性检测试剂盒

Cat:

NA0550

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/96S

Storage instructions：

-20℃

Product Overview：

Components:

Extract solution: 60 mL×1. Storage at 4℃.

Reagent IA: 14 mL×1. Storage at 4℃.

Reagent IB: Powder×1. Storage at -20℃.

Reagent IC: 1 mL×1. Storage at 4℃.

Reagent IIA: 3 mL×1. Storage at 4℃.

Reagent IIB: Powder×1. Storage at -20℃.

Reagent IIC: Powder×1. Storage at -20℃.

Reagent III: 20 mL×1. Storage at 4℃.

Preparation of solution：

Extract solution: Contains insoluble substance. Shake well before use.

Preparation of Reagent I: Add reagent 1B and reagent 1C to reagent 1A and dissolve thoroughly before

use. Separately store at -20℃ for 1 month.

Reagent IIA: Add 0.3ml distilled water to dissolve the reagent before use, and store the inexhaustible

reagent separately at -20℃ for two weeks.

Reagent IIB: Add 1ml distilled water to dissolve the reagent before use, and store the inexhaustible reagent

separately at -20℃ for two weeks.

Preparation of Reagent II: 1.305ml of reagent A, 0.12ml of reagent B and 0.075ml of reagent C were

mixed (1.5mL in total, about 75T) before use.

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Unit:

Price: $306

Product PDFs

Datasheet:

Other Information
Citations
Protein Attributes
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Pyruvate Decarboxylase (PDC) exists in yeast mainly, which is one of the key enzymes in ethanol

fermentation.

PDC catalyzes pyruvate decarboxylation to form acetaldehyde. The addition of alcohol dehydrogenase

(ADH) can further catalyze the reduction of aldehydes by NADH to ethanol and NAD⁺. NADH has an

absorbance at 340 nm but NAD⁺not, the activity of PDC can be calculated by measuring decrease rate of

absorption at 340 nm.

Reagents and Equipment Required but Not Provided:

Spectrophotometer/microplate reader, micro quartz cuvette/ 96 well flat-bottom plate (UV plate), water-

bath, desk centrifuge, adjustable pipette, mortar/homogenizer, ice and distilled water.

Protocol:

I. Sample extraction:

1. Bacteria:

Suggested 5 million bacteria/cell with 1 mL of Extract solution. Splitting bacteria/cell with ultrasonic (ice

bath, power 20%, work time 3s, interval 10s, repeat for 30 times). Centrifuge at 16000g for 20 minutes at 4

℃, take the supernatant and place it on ice for test.

2. Tissue:

Add 1 mL of Extract solution into 0.1 g of tissue, fully grinding on ice. Centrifuge at 16000 ×g at 4℃ for

20 minutes, take the supernatant and place it on ice for test.

3. Serum:

Detect directly.

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