XOD (EC 1.17.3.2) catalyzes the oxidation of xanthine to uric acid and superoxide anion, which is one of
the main sources of active oxygen and is also one of the key enzymes of nucleotide metabolism. XOD is
mainly distributed in mammalian heart, lung, liver and other tissues. When liver function impaired, XOD
is released into serum in a large amount, which has specific significance for the diagnosis of liver damage.
XOD catalyzes the production of uric acid from jaundice, which has a characteristic absorption peak at 290
nm.
Reagents and Equipment Required but Not Provided:
Spectrophotometer, centrifuge, adjustable pipette, water bath, 1 mL quartz cuvette, mortar/homogenizer,
ice and distilled water.
Sample preparation:
A. Bacteria or cells
Collecting bacteria or cells into the centrifuge tube, after centrifugation discard supernatant.
Accordance ratio bacteria or cell amount (104): Extractsolution volume(mL)=500~1000:1. Suggested
5 million with 1mL of Extract solution. Use ultrasonic to splitting bacteria or cell (placed on ice,
powder 20% or 200W, work time 3s，interval 10s，repeat for 30 times). Centrifuge at 8000g for 10
min at 4℃. The supernatant is placed on ice for test.
B. Tissue
Accordance ratio tissue weight(g) : Extract solution volume(mL)=1：5~10. Suggested 0.1g of tissue
with 1mL of Extract solution. Fully grind on ice, centrifuge at 8000g for 10 min at 4℃. Supernatant is
placed on ice for test. Or directly use 0.5mg/mL enzyme solution for direct measurement. To ensure
the accuracy of the experiment, it is recommended to use a gradient dilution of the extraction solution
for determination.
C. Serum (plasma) sample
Detect sample directly.
