NADP Phosphatase(NADPase) Activity Assay Kit

Home > Product > Test Kit > NADP Phosphatase(NADPase) Activity Assay Kit

NADP磷酸酶(NADPase)活性检测试剂盒

Cat:

NA0546

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/48S

Storage instructions：

2-8℃

Product Overview：

Components:

Extract solution: 50 mL ×1. Storage at 4℃.

Reagent I: 15 mL×1. Storage at 4℃.

Reagent II: Powder×2. Storage at 4℃. Add 1.1 mL of Reagent I to fully dissolve for later use and prepare

when the solution will be used.

Reagent III: Powder×1. Storage at 4℃. Add 8 mL ofdistilled water for use, dissolves and storage at 4 ℃

for a week.

ReagentIV: Powder×1. Storage at 4℃. Add 8 mL ofdistilled water for use, dissolves and storage at 4 ℃

for a week.

Reagent V: 8 mL×1. Storage at room temperature.

Standard: 1 mL×1.10 mmol/L standard phosphorus stock solution, Storage at 4℃.

Preparation of 1 μmol/mL standard phosphorus application solution: dilute the standard for 10 times,

namely 9 mL of distilled water is added into 1 mL of standard solution plus, fully mix.

Preparation of phosphorus detect reagent: it is prepared that the ratio of distilled water: Reagent III :

Reagent IV : Reagent V =2:1:1:1. The prepared phosphorus detect reagent should be light yellow. If it is

colorless, the reagent is invalid. If it is blue, it is polluted by phosphorus. Phosphorus detect reagent is

prepared when the solution will be used.

Note: it is better to use the new beaker, glass rod and glass pipette to prepare reagent, or to use disposable

plastic utensils to avoid phosphorus pollution.

prev next

Unit:

Price: $218

Product PDFs

Datasheet:

NADPase mainly exists in plant tissues and is the only enzyme in organisms that catalyzes the degradation

of NADP⁺to NAD⁺. NADPase and NADK regulate the balance between NAD and NADP.

NADPase can catalyze the hydrolysis of NADP⁺to NAD⁺and inorganic phosphorus, the activity of

NADPaseis determined by determining the content of inorganic phosphorus.

Reagents and Equipment Required but Not Provided

Refrigerated centrifuge, water-bath, adjustable pipette, spectrophotometer/microplate reader, micro

glasscuvette/96 well plate, homogenizer/mortar and distilled water.

Product Feedback Wall