Glutathione is a natural tripeptide composed of glutamic acid (Glu), cysteine (Cys) and glycine (Gly). It
is a kind of compound containing sulfhydryl group (-SH), which widely exists in animal tissue, plant
tissue, microorganism and yeast. Glutathione can react with 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) to
produce 2-nitro-5-mercaptobenzoic acid and glutathione disulfide (GSSG). 2-nitro-5-mercaptobenzoic acid
is a yellow product with the maximum absorption at 412 nm.
Technical Specifications
Minimum Detection Limit：2.67 μg/mL
Linear Range：3.125-250 μg/mL
Reagents and Equipment Required but Not Provided
Analytical balance, mortar/homogenizer, low temperature centrifuge, water bath, adjustable pipette,
spectrophotometer, 1 mL glass cuvette and distilled water.
