Glutathione is a natural tripeptide composed of glutamic acid (Glu), cysteine (Cys) and glycine (Gly).
It is a kind of compound containing sulfhydryl group (-SH), which widely exists in animal tissue, plant
tissue, microorganism and yeast. Glutathione can react with 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB)
to produce 2-nitro-5-mercaptobenzoic acid and glutathione disulfide (GSSG). 2-nitro-5-mercaptobenzoic
acid is a yellow product with the maximum absorption at 412 nm.
Technical Specifications
Minimum Detection Limit：3.763 μg/mL
Linear Range：12.5-400 μg/mL
Reagents and Equipment Required but Not Provided
Analytical balance, mortar/homogenizer, low temperature centrifuge, water bath, adjustable pipette,
spectrophotometer/microplate reader, micro glass cuvette or 96 well flat-bottom plate and distilled water.
