Oxidized Glutathione(GSSG) is an oxidized form of glutathione (GSH), also known as dithione
glutathione, which formed by the oxidation of two molecules of glutathione. GSSG is reduced to GSH by
glutathione reductase, so most of the body is in the reduced form. The determination of GSH and GSSG
content and ratio of GSH/GSSG in cells can reflect the redox status of cells. This kit utilizes reaction of
glutathione and 5,5'-dithiobis-2-nitrobenoic acid (DTNB) to produce 5-thio-2-nitrobenzoic acid. 5-thio-2-
nitrobenzoic acid has the largest absorption at wavelength of 412 nm, and 2-Vinylpyridine inhibit reduced
glutathione in the original of samples, and then using glutathione reductase to reduce GSSG to GSH,
determining the content of Oxidized Glutathione.
Technical Specifications
Minimum Detection Limit：1.369μg/mL
Linear Range：1.5625-50μg/mL
Reagents and Equipment Required but Not Provided
Analytical balance, mortar/homogenizer, centrifuge, water bath,adjustable pipette, spectrophotometer，1
mL glass cuvette and distilled water.
