Glutathione Peroxidase(GPX) Activity Assay Kit

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谷胱甘肽过氧化物酶(GSH-Px/GPX)活性检测试剂盒

Cat:

NA0779

Assay Type:

Spectrophotometer

Brand:

sunlong medical

Specificity:

50T/48S

Storage instructions：

2-8℃

Product Overview：

Components:

Extract solution: 30 mL×1, Storage at 4℃.

Reagent I: Powder×2, Storage at 4℃. Add 3.3 mL of Diluent to dissolve when the solution will be used.

Reagent II: 10 μL×1, Storage at 4℃. Add the liquid to the EP tube inside the reagent bottle. Dilute reagent

II with the ratio of 2μL reagent II and 10 mL distilled water before use.

Reagent III: 60 mL×1, Storage at 4℃. If the bottom of the bottle is crystallized, it can be dissolved in

water bath at 50℃. This solution is a saturated solution. If the bottom of the bottle is still crystallized, the

supernatant can be absorbed and used.

Reagent IV: 30 mL×1, Storage at 4℃.

Reagent V: 10mL×1, Storage at 4℃.

Standard: Powder×1, Storage at 4℃. 10 mg reduced glutathione (GSH). Add 0.405 mL of distilled water

to the standard solution of 80 μmol/mL when the solution will be used.

Diluent: 20 mL×1, Storage at 4℃.

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Unit:

Price: $220

Product PDFs

Datasheet:

Glutathione peroxidase (glutathione peroxidase, GSH-Px or GPX) is an important peroxidase widely

existed in the body. GPX can catalyzes the formation of oxidized glutathione (GSSG) from reduced

glutathione (GSH) and reduce toxic hydrogen peroxide to non-toxic hydroxyl compounds.

GPX catalyzes the oxidation of GSH by hydrogen peroxide to produce GSSG. GSH can react with DTNB

to form compounds with characteristic absorption peaks at 412 nm. The decrease of absorbance at 412 nm

can reflect the activity of GPX.

Reagents and Equipment Required but Not Provided:

Spectrophotometer, balance, table centrifuge, 1 mL glass cuvette, mortar/homogenizer, EP tube.

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