L-galactose way is the key for the synthesis of AsA. Gal LDH is located in the mitochondrial inner
membrane and is responsible for the final step of catalyzing the biosynthesis of AsA in plants. Gal LDH is
one of the key enzymes in this pathway. It plays an important role in the accumulation of AsA in plants.
Gal LDH catalyzes the reduction of L-galactonolactone to oxidized cytochrome c (Cyt c). Reduced Cyt c
has an absorption peak at 550 nm. Measure the increasing rate of reduced Cyt c can calculate the activity
of Gal LDH.
Reagents and Equipment Required but Not Provided:
Spectrophotometer/microplate reader, micro glass cuvette/96 well flat-bottom plate, desk centrifuge,
adjustable pipette, mortar/homogenizer, ice and distilled water.
