Protein carbonyl group is an early sign of various amino acids in the process of oxidative modification of
proteins. The carbonyl content of protein can indicate the degree of oxidative damage of protein, and it is
the main index to measure the oxidative damage of protein.
Carbonyl group can react with 2,4-dinitrophenylhydrazine to form red 2,4-dinitrophenylhydrazone with
characteristic absorption peak at 370 nm.
Reagents and Equipment Required but Not Provided:
Analysis balance, constant temperature water bath, centrifuge, vortex mixer, spectrophotometer, 1 mL
quartz cuvette, distilled water, anhydrous ethanol and ethyl acetate.
