Protein carbonyl group is an early sign of various amino acids in the process of oxidative modification of
proteins. The carbonyl content of protein can indicate the degree of oxidative damage of protein, and it is
the main index to measure the oxidative damage of protein.
Carbonyl group can react with 2,4-dinitrophenylhydrazine to form red 2,4-dinitrophenylhydrazone with
characteristic absorption peak at 370 nm.
Required material:
Balance, constant temperature water bath, low temperature centrifuge, vortex mixer, Spectrophotometer/
microplate reader, micro quartz cuvette/96-well flat-bottom plate (UV), distilled water, anhydrous ethanol,
ethyl acetate.
