Uric Acid(UA) Content Assay Kit

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尿酸(UA)含量检测试剂盒

Cat:

NA0763

Assay Type:

Spectrophotometer

Brand:

sunlong medical

Specificity:

50T/24S

Storage instructions：

-20℃

Product Overview：

Components:

Extract: Liquid 50 mL ×1. Storage at 4℃.

Reagent I: Liquid 80 mL×1. Storage at 4℃.

Reagent Ⅱ: Powder ×2 bottle. Store at 4℃ and protect from light. Add 6 mL of Reagent I before use. Mix

thoroughly. The rest of reagent store at 4℃. It can be stored for one week at 4℃.

Reagent Ⅲ: Powder ×1 bottle. Store at 4℃ and protect from light. Add 12 mL of Reagent I before use. Mix

thoroughly. The rest of reagent store at 4℃. It can be stored for two week at 4℃.

Reagent Ⅳ: Powder ×1 bottle. Store at 4℃ and protect from light. Add 12 mL of Reagent I before use. Mix

thoroughly. The rest of reagent store at 4℃. It can be stored for two week at 4℃.

Reagent Ⅴ: Powder ×1 bottle. Store at -20℃ and protect from light. Add 12 mL of Reagent I before use.

Mix thoroughly. Keep the unused reagents in separate packages at -20℃. Avoid repeated freezing and

thawing. It can be stored for two week at -20℃.

Reagent Ⅵ: Powder ×1 bottle. Store at -20℃ and protect from light. Add 12 mL of Reagent I before use.

Mix thoroughly. Keep the unused reagents in separate packages at -20℃. Avoid repeated freezing and

thawing. It can be stored for two week at -20℃.

Standard solution: Liquid ×1, 5 µmol/mL uric acid solution, store at 4℃ and protect from light.

Working solution A: It is used for the detection of sample test tube, blank tube and standard tube. According

to the ratio of Reagent Ⅱ: Reagent Ⅲ: Reagent Ⅳ: Reagent Ⅴ: Reagent Ⅵ=1:1:1:1:2 to prepare when the

solution will be used. It is recommended to use it within 2 hours after matching.

Working solution B: It is used for the detection of sample control tube. According to the ratio of Reagent Ⅱ:

Reagent Ⅲ: Reagent Ⅳ: Reagent Ⅴ: Reagent I=1:1:1:1:2 to prepare when the solution will be used. It is

recommended to use it within 2 hours after matching.

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Unit:

Price: $97

Product PDFs

Datasheet:

Other Information
Citations
Protein Attributes
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Uric acid is the end product of purine metabolism. Disorders of purine metabolism, energy metabolism and

renal excretion of uric acid can cause the increase or decrease of plasma uric acid level. And then lead to a

variety of diseases such as ventilation, kidney disease, cardiovascular disease. Therefore, the determination

of uric acid content has an important guiding significance in clinical diagnosis.

Uricase can catalyze the decomposition of uric acid into allantoin, CO₂and H₂O₂. Then Fe²⁺in potassium

ferrocyanide is oxidized by H₂O₂to form Fe³⁺. Fe³⁺can further react with 4-aminoantipyrine and phenol to

form red Quinones, which has a characteristic absorption peak at 505 nm. The content of uric acid can be

calculated by measuring the absorbance value at 505 nm.

Reagents and Equipment Required but Not Provided.

Spectrophotometer, table centrifuge, water-bath, 1 mL glass cuvette, adjustable pipette, mortar/

homogenizer, EP tube, ice and distilled water.

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