Home > Product > Test Kit > Thiol Content Assay Kit (Non-Protein Sample)
非蛋白质巯基含量检测试剂盒
Cat:
NA0756
Assay Type:
Spectrophotometer
Brand:
sunlong medical
Specificity:
50T/24S
Storage instructions:
2-8℃
Product Overview:
Components:  
Extract I: Liquid 25 mL ×1. Storage at 4℃.  
Extract II: Liquid 25 mL ×1. Storage at 4℃.  
Reagent I: Liquid 50 mL ×1. Storage at 4℃.  
Reagent II: Powder ×1. Store at 4℃ and protect from light. Add 5 mL of anhydrous methanol before use.  
Mix thoroughly.  
Standard: Powder ×1, 10 mg of cysteine, store at 4℃. Add 1.65 mL of Extract solution to dissolve it into  
50 μmol/mL standard solution before use.  
Preparation of Extract solution: mix Extract I and Extract II according to the volume ratio of 1:1, prepare  
according to the number of samples and use up on the same day.  
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Unit:
Price: $
Product PDFs
Datasheet:


The sulfhydryl group in organism mainly includes nonprotein sulfhydryl group and protein sulfhydryl  
group. Sulfhydryl compounds have important detoxification function in vivo. It has very important  
physiological significance to the self-regulation of organism.  
The thiol group reacts with 5,5 '- dithio-bis-Nitrobenzoic Acid (DTNB) to form a yellow compound. It has  
a maximum absorption peak at 412 nm.  
Reagents and Equipment Required but Not Provided.  
Spectrophotometer, table centrifuge, water bath, adjustable pipette, 1 mL glass cuvette, methanol,  
mortar/homogenizer and distilled water.  
 
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