The sulfhydryl group in organism mainly includes nonprotein sulfhydryl group and protein sulfhydryl
group. Sulfhydryl compounds have important detoxification function in vivo. It has very important
physiological significance to the self-regulation of organism.
The thiol group reacts with 5,5 '- dithio-bis-Nitrobenzoic Acid (DTNB) to form a yellow compound. It has
a maximum absorption peak at 412 nm.
Reagents and Equipment Required but Not Provided.
Spectrophotometer, table centrifuge, water bath, adjustable pipette, 1 mL glass cuvette, methanol,
mortar/homogenizer and distilled water.