Thiol Content Assay Kit (Non-Protein Sample)

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非蛋白质巯基含量检测试剂盒

Cat:

NA0514

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/48S

Storage instructions：

2-8℃

Product Overview：

Components:

Extract I: Liquid 30 mL ×1. Storage at 4℃.

Extract II: Liquid 30 mL ×1. Storage at 4℃.

Reagent I: Liquid 30 mL ×1. Storage at 4℃.

Reagent II: Powder ×1. Store at 4℃ and protect from light. Add 2 mL of anhydrous methanol before use.

Mix thoroughly.

Standard: Powder ×1, 10 mg of cysteine, store at 4℃. Add 1.65 mL of Extract solution to dissolve it into

50 μmol/mL standard solution before use.

Preparation of Extract solution: mix Extract I and Extract II according to the volume ratio of 1:1, prepare

according to the number of samples and use up on the same day.

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Unit:

Price: $48

Product PDFs

Datasheet:

The sulfhydryl group in organism mainly includes non-protein sulfhydryl group and protein sulfhydryl

group. Sulfhydryl compounds have important detoxification function in vivo. It has very important

physiological significance to the self-regulation of organism.

The thiol group reacts with 5,5 '- dithio-bis-nitrobenzoic acid (DTNB) to form a yellow compound. It has a

maximum absorption peak at 412 nm.

Reagents and Equipment Required but Not Provided.

Spectrophotometer/microplate reader, table centrifuge, water bath, adjustable pipette, micro glass

cuvette/96 well flat-bottom plate, methanol, mortar/homogenizer and distilled water.

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