Soil Ammonium Nitrogen Content Assay Kit

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土壤铵态氮含量检测试剂盒

Cat:

NA0748

Assay Type:

Spectrophotometer

Brand:

sunlong medical

Specificity:

50T/48S

Storage instructions：

-20℃

Product Overview：

Components:

Extract solution: 50 mL×1, store at 4℃.

Reagent I A: 1 mL×1, store at 4℃ and protect from the light.

Reagent I B: 4 mL×1, store at 4℃ and protect from the light. Before use, add Reagent I A into Reagent I B

and mix it for standby, or prepared when the solution will be used in proportion.

Reagent II: 5 mL×1, store at 4℃ and protect from the light.

Standard: 1 mL×1, 100 μg/mL NH₄-N standard solution, store at 4℃.

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Unit:

Price: $158

Product PDFs

Datasheet:

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Soil ammonium nitrogen is an important component of soil available nitrogen, which can be directly

absorbed and utilized by plants. Ammonium nitrogen index plays an important role in agricultural

production.

In the strong alkaline medium, the ammonium nitrogen in the soil interacts with hypochlorite and phenol to

form indophenol blue dye, which has a characteristic absorption peak at 630 nm, and the absorption value

is directly proportional to the ammonium nitrogen content.

Required reagents and equipments:

Spectrophotometer, scale, centrifuge, 1 mL glass cuvette, oscillator, constant temperature water bath.

Protocal:

I. Sample treatment

The ratio of soil mass (g): extract solution volume (mL) is 1:5~10 (it is recommended to weigh about 0.1 g

of soil sample and add 1 mL of extract solution), extract it by shaking for 1 hours. Centrifugate at 10000×g

for 10 minutes at 25℃, take the supernatant and place it for test.

II. Measurement operation

1. Preheat spectrophotometer/microplate reader for 30 minutes, adjust wavelength to 630 nm, set zero

with distilled water.

2. Dilute 100 μg/mL nitrogen standard solution with distilled water to 2.5 μg/mL for standby.

3. Sampling table

Reagent name (μL)

Sample

Blank tube (B)

Standard tube (S)

Test tube (T)

400

Distilled water

Standard

400

Reagent I

Reagent II

Mix well and place at room temperature of 25℃ for 30 minutes.

460 460

Distilled water

460

After fully mixing, measure the absorption value at 630 nm, and record it as A_B, A_Sand A_T. Calculate

ΔA_S=A_S-A_B, ΔA_T=A_T-A_B.

III. Calculation:

NH₄-N (μg/g Soil)= ΔA_T÷ΔA_S×C_S×V_E÷W=2.5×ΔA_T÷ΔA_S÷W

C_S: Concentration of standard solution, 2.5 μg/mL;

V_E: Volume of extract solution, 1 mL;

W: Sample mass, g.

Note:

1. Soil after Air-drying or drying can easily cause changes in NH₄-N content, so it is recommended to use

fresh soil for determination. The samples should be sealed, transported and stored at 4°C after collection,

and the analysis should be completed within 3 days. Otherwise, it should be stored in small pieces at -20°C

(deep freezing) and can be stored for several weeks. When determining the NH₄-N content of deep

freezing, the temperature and time of thawing should be controlled. When thawing at room temperature,

the sample must be thawed, homogenized and extracted within 4 hours; if it is thawed at 4°C, the thawing

time should not exceed 48 hours.

2. If you want to compare the NH₄-N content between different samples, you need to dry the soil sample

and calculate it by dry weight before comparing.

3. When ΔA is greater than 0.7, it is recommended to dilute the sample with distilled water for

determination.

4. The Reagent I shall be stored at 4℃ and protect from light and cannot be used after discoloration.

Technical Specifications：

Minimum Detection Limit：0.0646 ug/mL

Linear Range：0.156-5 ug/mL