Urea (BUN) is the main product of human protein metabolism. Urea constitutes the majority of non-
protein nitrogen in blood. Blood urea nitrogen is one of the main indexes of renal function. This kit use
indophenol blue colorimetric method to test NH3-N product by urease hydrolysis. The concentration of
indophenol is proportional to urea nitrogen concentration.
Reagents and Equipment Required but Not Provided:
Spectrophotometer/microplate reader, balance, cryogenic centrifuge, mortar/homogenizer, micro glass
cuvette/ 96-well flat-bottom plate, constant temperature water bath pot.