Serum iron is the iron bound with transferrin in blood, which is often used to distinguish non-iron
deficiency anemia and iron-deficiency anemia.
Fe3+ is reduced by sodium sulfite to Fe2+, which reacts with 2,2-dipyridine-bipyridine, have an absorption
peak at 520 nm. According measure absorbance at 520 nm can reflect serum iron concentration.
Reagents and Equipment Required but Not Provided.
Spectrophotometer, centrifuge, glacial acetic acid, adjusted transferpettor, 1 mL glass cuvette, chloroform
and distilled water.
