Serum iron is the iron bound with transferrin in blood, which is often used to distinguish non-iron
deficiency anemia and iron-deficiency anemia
Fe3+ is reduced by sodium sulfite to Fe2+, which reacts with 2,2-dipyridine-bipyridine, have an absorption
peak at 520 nm. According measure absorbance at 520 nm can reflect serum iron concentration.
Reagents and Equipment Required but Not Provided.
Spectrophotometer/microplate reader, centrifuge, micro glass cuvette/96 well flat-bottom plate, glacial
acetic acid, adjusted transferpettor, chloroform and distilled water.