Starch branching enzyme(SBE) exists mainly in plants, is a key enzyme of amylopectin biosynthesis. The
determination of SBE activity is of great significance in the study of starch biosynthesis, selection of high-
quality crop varieties and genetic improvement of quality.
The complex formed by the combination of starch and iodine has a characteristic absorption peak at
660nm. SBE can cut off the side branches of amylopectin, thus reducing the absorption value of starch
iodine complex at 660 nm. In a certain time, the percentage of absorbance decrease can reflect SBE
activity.
Reagents and Equipments Required but Not Provided:
Spectrophotometer/microplate reader, water bath, desk centrifuge, adjustable transferpettor, micro glass
cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water.
