AchE is a serine hydrolytic enzyme, which is widely found in various animal tissues and serum. AchE
catalyzes the hydrolysis of Ach, which plays an important role in the regulation of nerve conduction.
AchE catalyzes Ach hydrolysis to generate choline, and choline can react with 2-nitrobenzoic acid
(DTNB) to form 5-mercapto nitrobenzoic acid (TNB). TNB has an absorption peak at 412 nm, and AchE
activity was calculated by measuring the absorbance increasing rate at 412 nm.
Reagents and Equipment Required but Not Provided.
Refrigerated centrifuge, water-bath, transferpettor, spectrophotometer/microplate reader, micro glass
cuvette/96 well flat-bottom plate, homogenizer/mortar and distilled water.
