Isocitrate Lyase(ICL) Activity Assay Kit

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异柠檬酸裂解酶（ICL）活性检测试剂盒

Cat:

NA0482

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/96S

Storage instructions：

-20℃

Product Overview：

Components:

Extract solution: Liquid100 mL×1. Storage at 4℃. Add reagent VII to the extract solution before use.

Reagent I: 3 mL×1. Storage at 4℃.

Reagent II: 4 mL×1. Storage at 4℃.

Reagent III: Powder×1. Storage at -20℃. Dissolve it thoroughly with 5mL of distilled water before use.

The reagents can be stored at - 20℃ after being packed separately. Avoid repeated freezing and thawing.

Reagent IV: Powder×1. Storage at -20℃. Dissolve it thoroughly with 5mL of distilled water before use.

The reagents can be stored at - 20℃ after being packed separately. Avoid repeated freezing and thawing.

Reagent V: Liquid 40 μL×1. Storage at 4℃. The liquid is placed in the EP tube in the reagent bottle.

According to the dosage and the volume ratio of Reagent V: distilled water=1:8, and the mixture is ready

to use.

Reagent VI: Powder×1. Storage at 4℃. Dissolve it thoroughly with 5 mL of distilled water before use. The

reagents can be stored at 4℃.

Reagent VII: Powder×1. Storage at 4℃.

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Unit:

Price: $328

Product PDFs

Datasheet:

Other Information
Citations
Protein Attributes
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ICL (ec4.1.3.1) mainly exists in plants and microorganisms. During the germination process of oil seeds,

fat is converted into carbohydrate through glyoxylate cycle and other processes. ICL is one of the key

enzymes in glyoxylate cycle.

The degradation of isocitric acid to glyoxylic acid and succinic acid is catalyzed by ICL. Glyoxylic acid

and NADH generate ethanol and NAD under the action of LDH. NADH has a characteristic absorption

peak at 340nm. Monitoring the reduction rate of 340nm absorbance can indirectly reflect the activity of

ICL.

Reagents and Equipment Required but Not Provided:

Spectrophotometer/ Microplate reader, water bath, desk centrifuge, adjustable transferpettor, micro quartz

cuvette/ 96 well flat-bottom plate, mortar / homogenizer, ice and distilled water.

Sample preparation:

1. Cells or bacteria: Collect bacteria or cells into centrifuge tube, after centrifugation discard supernatant.

Suggest 2 million of bacteria or cells with 0.4 mL of extract solution, splitting with ultrasonication (ice

bath, power 20%, work time 3s, interval 10s, for 30 times). Centrifuge at 15000 ×g for 10 minutes at 4℃

to remove insoluble materials and take the supernatant on ice before testing.

2. Tissue: Add 1 mL of extract solution into 0.1 g of tissue, fully grinding on ice. Centrifuge at 15000 ×g

for 10 minutes at 4℃ to remove insoluble materials and take the supernatant on ice before testing.

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