Phosphoenolpyruvate Carboxylase(PEPC) Activity Assay Kit

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磷酸烯醇式丙酮酸羧化酶(PEPC)活性检测试剂盒

Cat:

NA0715

Assay Type:

Spectrophotometer

Brand:

sunlong medical

Specificity:

50T/48S

Storage instructions：

-20℃

Product Overview：

Components:

Extract solution: Liquid 60 mL×1, store at 4℃;

Reagent I: Liquid 30 mL×1, store at 4℃;

Reagent II: Liquid 5 mL×1, store at 4℃.

Reagent III: Liquid 5 mL×1, store at 4℃.

Reagent IV: Powder ×1, store at -20℃. Add 4 mL of distilled water before use; Mix thoroughly. It can be

stored at -20℃ after sub packaging. Avoid repeated freezing and thawing;

Reagent V: Powder ×1, store at -20℃. Add 4 mL of distilled water before use; Mix thoroughly. It can be

stored at -20℃ after sub packaging. Avoid repeated freezing and thawing;

Reagent VI original solution: Liquid 25 μL×1, store at 4℃;

Reagent VI diluent: Liquid 10 mL×1, store at 4℃;

Reagent VII: Powder ×1, store at -20℃. Add 5 mL of double distilled water before use; Mix thoroughly. It

can be stored at -20℃ after sub packaging. Avoid repeated freezing and thawing;

Reagent VI working solution: Dilute the original solution of reagent VI: diluent of reagent VI in the

proportion of 7.5 μL: 322.5 μL. Match the solution as much as you use.

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Unit:

Price: $185

Product PDFs

Datasheet:

Other Information
Citations
Protein Attributes
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Phosphoenolpyruvate carboxylase (EC 4.1.1.31) is widely found in plants and microorganisms. It catalyzes

the irreversible reaction of phosphoenolpyruvate and carbon dioxide to oxaloacetic acid. It is also a key

enzyme for C4 plants and cam plants to fix CO₂. It plays an important role in regulating the operation of

tricarboxylic acid cycle.

PEPC can catalyze phosphoenolpyruvate and carbon dioxide to form oxaloacetate and HPO₄. Malate

dehydrogenase can catalyze oxaloacetate and NADH to produce malate and NAD⁺. The rate of NADH

reduction was measured at 340 nm and PEPC activity was calculated.

Required but Not Provided:

Ultraviolet spectrophotometer, desk centrifuge, water-bath, transferpettor, 1 mL quartz cuvette, pipette,

mortar/homogenizer, ice and distilled water.

Protocol

I. Preparation:

1. Tissue: according to the tissue weight (g): the Extract solution volume (mL) is 1:5-10. (It is

recommended that add 1 mL of Extract solution to 0.1 g tissue). Homogenate in ice bath, then centrifuge at

8000 g for 20 minutes at 4℃. Take the supernatant for test.

2. Cells: according to the number of the cells (10⁴): the volume of the Extract solution (mL) is 500~1000:1.

It is suggested that add 1 mL of Extract solution to 500 million of cells. Breaking cells by ultrasonic wave

in ice bath (power 300W, ultrasonic 3s, interval 7s, total time 3 min). Centrifuge at 8000 g 4℃ for 20

minutes. Take the supernatant on ice for test.

II. Determination

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