Pyruvate connects glucose, fatty acid and amino acid metabolism through acetyl CoA and plays an
important pivotal role.
Pyruvate reacts with 2, 4-dinitrophenylhydrazine to produce pyruvate-2, 4-dinitrophenylhydrazone, which
is fuchsia-red in alkaline solution.
Reagents and Equipment Required but Not Provided.
Table centrifuge, water-bath, spectrophotometer, 1 mL glass cuvette, transferpettor, mortar/homogenizer,
ice and distilled water.
