Acyltransferases are a large family of multifunctional proteins, which are mainly responsible for catalyzing
various acylation and deacylation reactions in the body, playing an important role in gene expression,
metabolism and signaling.
Acyltransferase catalyzes acetyl CoA to transfer acetyl to butanol, and at the same time reduces DTNB to
generate TNB; TNB has an absorption peak at 412 nm, and the rate of increase in absorbance at 412 nm is
measured to calculate Acyltransferase activity.
Reagents and Equipment Required but Not Provided.
Spectrophotometer, analytical balance, 1 mL glass cuvette, distilled water, centrifuge, water bath,
adjustable pipette, mortar/homogenizer, ice and distilled water
Sample pre-treatment:
Tissue sample: Weigh about 0.1 g of sample and add 1 mL of Extraction reagent, fully grind on ice,
centrifuge at 15000 rpm at 4°C for 20 min, and the supernatant is to be tested.
Serum samples: directly detect.
