Glucose is not only the main substrate of cell energy metabolism, but also its metabolic intermediate is an
important substrate of biosynthesis. Plants produce glucose through photosynthesis. In mammals, glucose
is not only the sole source of energy for the nervous system, muscles and adipose tissue of the brain, but
also is closely related to the synthesis of reductive coenzymes, lactose and milk fat.
Glucose oxidase catalyzes the oxidation of glucose to gluconic acid and hydrogen peroxide. Peroxidase
catalyzes the oxidation of 4-aminoantipyrine bisphenol by hydrogen peroxide to form colored compounds
with characteristic absorption peaks at 505 nm.
Reagents and Equipment Required but Not Provided.
Water-bath, transferpettor, spectrophotometer/microplate reader, micro glass cuvette/96 well flat-bottom
plate, mortar/homogenizer and distilled water.
