Trehalase（THL）Activity Assay Kit

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海藻糖酶（THL）活性检测试剂盒

Cat:

NA0691

Assay Type:

Spectrophotometer

Brand:

sunlong medical

Specificity:

50T/24S

Storage instructions：

2-8℃

Product Overview：

Components:

Extract solution: Liquid 50 mL×1. Storage at 4℃.

Reagent I: Liquid 30 mL×1. Storage at 4℃.

Reagent II: Powder×1. Storage at 4℃. Dissolve with 2.5 mL of Reagent I before use. Prepare when the

solution will be used.

Reagent III: Liquid 30 mL×1. Storage at 4℃.

Reagent IV: Liquid 30 mL×1. Storage at room temperature.

Standard: Powder×1. Storage at 4℃. Contain 10 mg of anhydrous glucose (dry weight loss < 0.2%).

Dissolve the standard with 1 mL of distilled water to generate a 10 mg/mL glucose solution standard, store

at 4 ℃ and use within one week. Or it can be dissolved in saturated benzoic acid solution for a longer time.

Standard solution: Dilute the 10 mg/mL glucose solution standard to 1, 0.8, 0.6, 0.4, 0.2 mg/mL with

distilled water.

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Unit:

Price: $114

Product PDFs

Datasheet:

Trehalase(THL, EC 3.2.1.28) is an enzyme found broadly in animals, plants, microorganisms and cultured

cells.The main function of THL is to break down trehalose to produce glucose and directly use it for the

energy supply of organism.

THL converts soluble trehalose to reducing sugar. 3.5-Dinitrosalicylic acid is reduced to brown-red amino

compound by co-heating with reducing sugar. The brown-red amino compound has a maximum

absorption peak at 540 nm and the absorbance ratio is in direct proportion to the contents of reducing

sugar. In this kit, the THL activity is quantified by measuring the color development at 540 nm.

Reagents and Equipments Required but Not Provided:

Spectrophotometer, water bath, desk centrifuge, adjustable transferpettor, 1 mL glass cuvette,

mortar/homogenizer, ice and distilled water.

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