Trehalase（THL）Activity Assay Kit

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海藻糖酶（THL）活性检测试剂盒

Cat:

NA0450

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/48S

Storage instructions：

2-8℃

Product Overview：

Components:

Extract solution: Liquid 100 mL×1. Storage at 4℃.

Reagent I: Liquid 10 mL×1. Storage at 4℃.

Reagent II: Powder×1. Storage at 4℃. Dissolve with 2 mL of Reagent I before use. Prepare when the

solution will be used.

Reagent III: Liquid 13 mL×1. Storage at 4℃.

Reagent IV: Liquid 13 mL×1. Storage at room temperature.

Standard: Powder×1. Contain 10 mg of anhydrous glucose (dry weight loss < 0.2%). Dissolve the standard

with 1 mL of distilled water to generate a 10 mg/mL glucose solution standard, store at 4 ℃ and use within

one week. Or it can be dissolved in saturated benzoic acid solution for a longer time.

Standard solution: Dilute the 10 mg/mL glucose solution standard to 1, 0.8, 0.6, 0.4, 0.2 mg/mL with

distilled water.

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Unit:

Price: $233

Product PDFs

Datasheet:

Trehalase(THL, EC 3.2.1.28) is an enzyme found broadly in animals, plants, microorganisms and cultured

cells.The main function of THL is to break down trehalose to produce glucose and directly use it for the

energy supply of organism.

THL converts soluble trehalose to reducing sugar. 3.5-Dinitrosalicylic acid is reduced to brown-red amino

compound by co-heating with reducing sugar. The brown-red amino compound has a maximum

absorption peak at 540 nm and the absorbance ratio is in direct proportion to the contents of reducing

sugar. In this kit, the THL activity is quantified by measuring the color development at 540 nm.

Reagents and Equipments Required but Not Provided:

Spectrophotometer/microplate reader, water bath, desk centrifuge, adjustable transferpettor, micro glass

cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water.

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