Cellulase (EC 3.2.1.4) exists in bacteria, fungi and animals，which can catalyze cellulose degradation. It is
a type of enzyme preparation that can be widely used in the fields of medicine, food, cotton spinning,
environmental protection and renewable resource utilization.
The 3.5-dinitrosalicylic acid method is used to determine the reducing sugar content of cellulose catalyzed
by CL.
Reagents and Equipment Required but Not Provided:
Spectrophotometer, adjustable transferpettor, balance, mortar/homogenizer, centrifuge, 1mL glass cuvette,
ice and distilled water.
Sample preparation:
1. Bacteria or cells: Collect the bacteria or cells into a centrifuge tube, discard the supernatant after
centrifugation; add 1 mL of Extract reagent for every 5 million bacteria or cells, and break the bacteria
or cells with an ultrasonic ice bath (power 20%, ultrasonic 3 seconds, interval 10 seconds, repeat 30
times); Centrifugate at 8000g and 4 ℃ for 10min, take the supernatant and place on ice for testing.
2. Plant and animal tissues: Weigh about 0.1 g of sample, add 1 mL of Extract reagent and fully grind.
Centrifugate at 8000g and 4℃ for 10 min, the supernatants as samples to be tested.
