Pectinase is one of the enzymes that decompose pectin, including protopectinase, pectinesterase,
polygalacturonase and pectinase. It widely exists in fruits of higher plants and microorganisms and is the
most important enzyme in fruit processing.
Pectinase hydrolyzes pectin to produce galacturonic acid, which reacts with DNS reagent to produce
brownish red substance with characteristic absorption peak at 540 nm. The activity of pectinase can be
calculated by measuring the change of absorption value at 540 nm.
Reagents and Equipment Required but Not Provided
Spectrophotometer/microplate reader, table type centrifuge, water bath, micro glass cuvette/96 well flat-
bottom plate, adjustable pipette, mortar/homogenizer, ice and distilled water.
