Serum zinc is one of the essential trace elements, which also plays an important role in insulin and
porphyrin metabolism. In the solution of pH 8.5~9.5, the complex produced by Zn2+ and zinc reagent has a
maximum absorption peak at 620 nm.
Reagents and Equipment Required but Not Provided.
Spectrophotometer/microplate reader, centrifuge, pipette, micro glass cuvette/96 well flat-bottom plate,
vortex mixer /magnetic stirrer, distilled water.