Inorganic phosphorus mainly refers to phosphate radical, which is involved in many kinds of metabolism,
including energy metabolism, nucleic acid metabolism, protein phosphorylation and dephosphorylation,
and so on. In addition, inorganic phosphorus promotes the synthesis, transformation and transport of
carbohydrates.
Molybdenum blue can react with phosphate group, the reaction product can be detected by colorimetric
assay at 660 nm and calculate the inorganic phosphorus content indirectly.
Required material
Spectrophotometer/microplate reader, centrifuge, water bath, transferpettor, micro glass cuvette/96 well
flat-bottom plate, distilled water.
