Soil hydroxylamine reductase can reduce the intermediate product hydroxylamine formed in the process of
soil nitrogen metabolism to ammonia, and the reduced compounds in the soil can be used as hydrogen
donors. Its strength affects the ammonia volatilization loss of nitrogen in the process of soil nitrogen
metabolism, and indirectly affects the utilization efficiency of nitrogen.
Fe3 + in ammonium ferric sulfate can oxidize hydroxylamine to nitrogen and reduce itself to Fe2+, Fe2+
forms orange red complex with o-phenanthroline under weak acid condition, orange red complex has
absorption peak at 510nm. hydroxylamine reductase acts on hydroxylamine that could reduce the amount
of formation of complex, and the decrease of absorption value at 510nm can reflect the activity of
hydroxylamine reductase.
Reagents and Equipment Required but Not Provided
Spectrophotometer, scales, centrifuge, transferpettor, 1 mL glass cuvette, vortex shaker, nitrogen blower,
EP tube, sieve (30-50 mesh) and distilled water.
