S-α-GC can catalyze the hydrolysis of glycosidic bonds between aryl or hydrocarbon groups and glycosyl
groups to form glucose. It is one of the important components of cellulolytic enzyme system. It has
important physiological functions in carbohydrate metabolism of soil microorganisms.
S-a-GC can catalyze the formation of p-nitrophenol from p-nitrobenzene-a-d-glucopyranoside. It has
characteristic light absorption at 400 nm.
Required but Not Provided:
Spectrophotometer, desk centrifuge, water-bath, transferpettor, 1 mL glass cuvette, 50 mesh sieve(or
smaller), toluene (express delivery is not allowed) and distilled water.
Protocol
I. Preparation:
The fresh soil sample is dried by natural air or air in a 37℃ oven, and passes through 30-50 mesh sieve.
II. Determination