Soil α-glucosidase(S-α-GC) Activity Assay Kit

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土壤α-葡萄糖苷酶（S-α-GC）活性检测试剂盒

Cat:

NA0404

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/48S

Storage instructions：

-20℃

Product Overview：

Components:

Reagent I: Toluene 0.5 mL×1, store at 4℃; Self-provided reagent;

Reagent II: Powder×2, store at -20℃. Add 5 mL distilled water when the solution will be used. Mix

thoroughly. The rest of reagent store at -20℃;

Reagent III: Liquid 15 mL×1, store at 4℃;

Reagent IV: Liquid 30 mL×1, store at 4℃;

Standard solution: Liquid 1 mL×1, 5 mmol/L of p-nitrophenol solution. Dilute the standard 50 times with

reagent III before use to obtain 100 μmol/L standard solution.

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Unit:

Price: $220

Product PDFs

Datasheet:

S-α-GC can catalyze the hydrolysis of glycosidic bonds between aryl or hydrocarbon groups and glycosyl

groups to form glucose. It is one of the important components of cellulolytic enzyme system. It has

important physiological functions in carbohydrate metabolism of soil microorganisms.

S-a-GC can catalyze the formation of p-nitrophenol from p-nitrobenzene-a-d-glucopyranoside. It has

characteristic light absorption at 400 nm.

Required but Not Provided:

Spectrophotometer/microplate reader, desk centrifuge, water-bath, transferpettor, micro glass cuvette/96

well flat-bottom plate, 50 mesh sieve (or smaller), toluene (express delivery is not allowed) and distilled

water.

Protocol

I. Preparation:

The fresh soil sample is dried by natural air or air in a 37℃ oven, and passes through 30-50 mesh sieve.

II. Determination

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