Phosphoenolpyruvate Carboxykinase(PEPCK) Activity Assay Kit

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磷酸烯醇式丙酮酸羧激酶（PEPCK）活性检测试剂盒

Cat:

NA0635

Assay Type:

Spectrophotometer

Brand:

sunlong medical

Specificity:

50T/48S

Storage instructions：

-20℃

Product Overview：

Components:

Extract solution: 60 mL ×1. Storage at 4℃.

Reagent I: 45 mL×1, stored at 4℃.

Reagent II: powder×1, stored at -20℃ and protected from light. Dissolve it in 35 mL of Reagent I before

use. The reagent that cannot be used up shall be stored at -20℃ after repacking, repeat freezing and

thawing are prohibited;

Reagent III: 45 μL×1, stored at 4℃ and protected from light. Before temporary use, add distilled water to

dilute according to the volume ratio of 1:120, prepare when the solution will be used.

Reagent IV: 155 μL×1, stored at 4℃ and protected from light. Before temporary use, add distilled water

to dilute according to the volume ratio of 7:250, prepare when the solution will be used.

Reagent V: Powder×1, stored at -20℃ and protected from light. Dissolve it in 2.5 mL of distilled water

before use, the reagent that cannot be used up shall be stored at -20℃ after repacking, repeat freezing and

thawing are prohibited;

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Unit:

Price: $282

Product PDFs

Datasheet:

PEPCK (EC 4.1.1.32) is widely found in animals, flowering plants, algae, some fungi and bacteria. The

enzyme catalyzes the conversion of oxaloacetic acid to phosphoenolpyruvate, which is the first-rate

limiting enzyme regulating gluconeogenesis.

PEPCK catalyzes oxaloacetic acid to form phosphoenolpyruvate and CO₂, pyruvate kinase and lactate

dehydrogenase further catalyze the oxidation of NADH to NAD⁺in turn, and determine the NADH

decline rate at 340 nm, which can reflect the PEPCK activity.

Reagents and Equipment Required but Not Provided

Spectrophotometer, low temperature centrifuge, water bath pot, 1 mL quartz cuvette, adjustable pipette,

mortar/homogenizer, ice and distilled water

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