Glucose-6-phosphatase (G6Pase, EC 3.1.3.9) is a kind of phosphatase which hydrolyzes phosphate
compounds. It widely exists in animals, plants, microorganisms and cells. It is a restriction enzyme which
hydrolyzes glucose-6-phosphate to produce glucose in the process of gluconeogenesis. It plays an
important role in maintaining the dynamic balance of blood glucose.
G6P catalyzes glucose-6-phosphate to produce glucose and inorganic phosphorus. The increase of
inorganic phosphorus content by molybdenum blue method can reflect the activity of G6P.
Reagents and Equipment Required but Not Provided:
Visible spectrophotometer, low temperature desktop centrifuge, water bath pot, 1 mL glass cuvette,
adjustable pipette, mortar/homogenizer, EP tube, ice and distilled water.
