Glycogen synthase(GCS) Activity Assay Kit

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糖原合成酶（GCS）检测试剂盒

Cat:

NA0391

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/96S

Storage instructions：

-20℃

Product Overview：

Components:

Extract solution: Liquid 100 mL×1, store at 4℃;

Reagent I: Liquid 18 mL×1, store at 4℃;

Reagent II: Liquid 7.5 mL×1, store at 4℃;

Reagent III: Liquid 14 μL×1, store at 4℃ and protect from light;

Reagent IV: Powder×1, store at -20℃;

Reagent V: Powder×1, store at -20℃;

Reagent VI: Liquid 48 μL×1, store at 4℃ and protect from light;

Reagent VII: Powder×1, store at -20℃;

Reagent VIII: Powder×1, store at 4℃ and protect from light;

Working solution: Transfer reagent III, IV and V to reagent I for mixing and dissolving before use; The

rest of reagent can store at -20℃ for one week; Avoid repeated freezing and thawing;

Preparation of reagent VIII: Add 5 mL of reagent II into reagent VIII and dissolve it before use. Then

transfer reagent VI and VII to reagent VIII to mix and dissolve them for use; The rest of reagent can store

at -20℃ for one week; Avoid repeated freezing and thawing;

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Unit:

Price: $627

Product PDFs

Datasheet:

Other Information
Citations
Protein Attributes
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Glycogen synthetase (GCS) can add the glycogen of UDPG to the original glycogen or the non-reducing

end of glycogen protein, and be connected by α-1,4 glycoside bond. GCS is the rate limiting enzyme of

glycogen synthesis in animal body. It is also the main target enzyme of insulin. It plays an important role

in the process of glucose metabolism and maintaining the relative stability of blood glucose.

GCS catalyzes the production of glycogen and UDP from UDPG and glucose residues. Pyruvate kinase

and lactate dehydrogenase further catalyze NADH to generate NAD⁺. The decrease rate of NADH at 340

nm can reflect the activity of GCS.

Required but Not Provided:

Ultraviolet spectrophotometer/microplate reader, balance, low temperature desk centrifuge, water-bath,

transferpettor, micro quartz cuvette/96 well flat-bottom UV plate, EP tube, mortar/homogenizer, ice and

distilled water.

Protocol

I. Preparation:

1. Tissue: according to the ratio of mass (g): extraction volume (mL): 1:5-10 to add the extract. It is

suggested that add 1 mL of extract to 0.1 g of tissue. Homogenate on ice. Centrifuge at 8000 g 4℃ for 10

min. Take the supernatant on ice for test.

2. Bacteria and cells: according to the ratio of 10⁴cells: extract volume (mL) 500-1000:1. It is suggested to

take about 500 million bacteria/cell and add 1 mL extraction reagent. Bacteria/cell is split by

ultrasonication (power 200w, ultrasonic 3s, interval 7s, total time 3 min). Centrifuge at 8000 g 4℃ for 10

min. Take the supernatant on ice for test.

3. Serum and other liquids: detect directly.

II. Determination

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