Glycogen Phosphorylase b (Gpb) Activity Assay Kit

Home > Product > Test Kit > Glycogen Phosphorylase b (Gpb) Activity Assay Kit

糖原磷酸化酶b（GPb）活性检测试剂盒

Cat:

NA0389

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/48S

Storage instructions：

-20℃

Product Overview：

Components:

Reagent

Extract solution

Reagent I

Size

Storage

4℃

60 mL×1

40 mL×1

Powder×1

Powder×2

Powder×1

4℃

Reagent II

4℃

Reagent III

Reagent IV

Reagent V

4℃

-20℃

4℃

Reagent VI

Reagent VII

Solution preparation:

1. Reagent II: Dissolved with 0.5 mL of distilled water before use. Mix thoroughly.

2. Reagent III: Dissolved with 0.5 mL of distilled water before use. Mix thoroughly. It can be divided

into small tubules and stored for 4 weeks at -20℃. Avoid repeating freeze/thaw cycles.

3. Reagent IV: Dissolved with 1.25 mL of distilled water before use. Mix thoroughly. It can be divided

into small tubules and stored for 4 weeks at -20℃. Avoid repeating freeze/thaw cycles.

4. Reagent V: Dissolved with 1 mL of distilled water before use. Mix thoroughly. It can be divided into

small tubules and stored for 4 weeks at -20℃. Avoid repeating freeze/thaw cycles.

5. Reagent VI: Dissolved with 1 mL of distilled water before use. Mix thoroughly. It can be divided into

small tubules and stored for 4 weeks at -20℃. Avoid repeating freeze/thaw cycles.

6. Reagent VII: Dissolved with 2 mL of distilled water before use. Mix thoroughly. It can be divided into

small tubules and stored for 4 weeks at -20℃. Avoid repeating freeze/thaw cycles.

7. Working solution: Calculate according to the amount required for the experiment before use, according

to the ratio of reagent I: reagent II: reagent III: reagent IV= 148μL: 4μL: 4μL: 4μL(amount of 1T), mix well

before use.

prev next

Unit:

Price: $462

Product PDFs

Datasheet:

Other Information
Citations
Protein Attributes
Feedback Wall

Glycogen phosphorylase is divided into active glycogen phosphorylase a (Glycogen phosphorylase a, GPa)

and inactive glycogen phosphorylase b (Glycogen phosphorylase b, GPb) two forms. The decomposition of

glycogen is mainly carried out under the catalysis of glycogen phosphorylase a. When no activator is added,

glycogen phosphorylase a catalyzes the production of glucose residues from glycogen

BC3355 -- page 1 / 4

and inorganic phosphorus to glycogen and glucose 1-phosphate. Under the action of phosphoglucose mutase

and 6-phosphate glucose dehydrogenase, it further catalyzes the reduction of NADP to NADPH. Measuring

the rate of increase of NADPH at 340nm can reflect the activity of glycogen phosphorylase a. The activity

of GP (GPa and GPb) is measured by adding an activator, and GPa activity is measured when no activator

is added. GPb activity can be obtained by subtracting GPa activity from GP activity.

Reagents and Equipment Required but Not Provided：

Ultraviolet spectrophotometer/microplate reader, low temperature centrifuge, constant temperature

incubator/water bath, adjustable pipette, mortar/homogenizer, micro quartz cuvette/96 well UV flat -bottom

plate, ice and distilled water.

Product Feedback Wall